Comparison of the phenotypic profile of antimicrobial resistance in the oral microbiota of non-smokers, tobacco smokers, and electronic cigarette vapers - A pilot study

Authors

  • Enrique Alejandro Martínez-Solís Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA) https://orcid.org/0000-0002-5563-8891
  • Abraham Atman Hernández-Martínez Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0003-2452-4119
  • Adán Peña-Barreto Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0002-7489-5324
  • Tomás Barrientos-Fortes Universidad Anáhuac México, Sistema Universitario de Salud https://orcid.org/0000-0001-6930-3710
  • Diego Abelardo Álvarez-Hernández Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0003-2340-6639
  • Sandra Georgina Solano-Gálvez  Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Microbiología https://orcid.org/0000-0003-0845-7200
  • Rosalino Vázquez-López Universidad Anáhuac México, Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Departamento de Microbiología https://orcid.org/0000-0002-1982-7329

DOI:

https://doi.org/10.36105/psrua.2021v1n1.01

Keywords:

cigarrillo, cigarrillos electrónicos, vapear, microbiota, resistencia a fármacos antimicrobianos

Abstract

Introduction: The microbiota is a community of microorganisms that live in a specific environment. Their type and number depend on multiple internal and external factors. Oral is the second most diverse and populated microbiota of the body. Smoking and vaping induce changes in its composition, and it has been demonstrated that they can lead to an increase in antimicrobial resistance. Objective: To compare the phenotypic profile of antimicrobial resistance in the oral microbiota of non-smokers, tobacco users, and electronic cigarette vapers. Methods: An observational, descriptive, cross-sectional, and comparative study was carried out. Three groups of non-smokers, smokers of conventional tobacco, and electronic cigarette (EC) vapers of tobacco flavored e-juice were formed. Oral cavity samples were obtained, incubated, and seeded in agar plates. Bacteria were isolated and identified performing Gram staining, oxidase, indole, and biochemical test panels. Susceptibility tests were performed using a MicroScan autoSCAN-4 system and the Kirby–Bauer test. Results: Variation was observed in the populations of bacteria that were isolated in each of the groups, but the non-smokers showed the most pathogens. In the non-smoking group, Staphylococcus sciuri was the most common bacteria, Staphylococcus sciuri and Enterobacter cloacae were the most abundant in the smoking group, and in the EC vapers group, the most common bacteria were Staphylococcus epidermidis and Staphylococcus sciuri. Conclusion: Multidrug resistance was observed in all the groups. However, EC vapers showed the highest proportions of antimicrobial resistance, raising a major concern.

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Published

2021-07-22

How to Cite

Martínez-Solís, E. A., Hernández-Martínez, A. A., Peña-Barreto, A., Barrientos-Fortes, T., Álvarez-Hernández, D. A., Solano-Gálvez, S. G., & Vázquez-López, R. (2021). Comparison of the phenotypic profile of antimicrobial resistance in the oral microbiota of non-smokers, tobacco smokers, and electronic cigarette vapers - A pilot study. Proceedings of Scientific Research Universidad Anáhuac. Multidisciplinary Journal of Healthcare, 1(1), 5–13. https://doi.org/10.36105/psrua.2021v1n1.01

Issue

Vol. 1 No. 1 (2021)

Section

Original Research

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Copyright (c) 2021 Enrique Alejandro Martínez-Solís, Abraham Atman Hernández-Martínez, Adán Peña-Barreto, Tomás Barrientos-Fortes, Diego Abelardo Álvarez-Hernández, Sandra Georgina Solano-Gálvez, Rosalino Vázquez-López

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